Allen and Arnon Medium plus Nitrate

ATCC Medium 1142

Solution A25.0 ml
Solution B6.25 ml
KNO30.253 g
NaNO30.212 g
Purified agar (if needed)10.0 g
Distilled water969.0 ml
Add Solution B aseptically after sterilization.


Solution A (A & A minus Phosphate concentrate):
4% MgSO4·7H2O500.0 ml
1.2% CaCl2·2H2O500.0 ml
3.8% NaCl500.0 ml
Microelements stock solution500.0 ml

Solution B (K2HPO2 Stock solution):
K2HPO228.0 g
Distilled water500.0 ml

Microelements stock solution:
A & A FeEDTA solution160.0 ml
MnCl2·4H2O360 mg
MoO336.0 mg
ZnSO2·7H2O44.0 mg
CuSO2·5H2O15.8 mg
H3BO3572.0 mg
NH4VO34.6 mg
CoCl2·6H2O8.0 mg
Distilled water1090.0 ml

A & A FeEDTA solution:
Dissolve 5.2 g KOH in 186 ml distilled water
Add 20.4 g Na2EDTA·2H2O
Dissolve 13.7 g FeSO4·7H2O in 364 ml distilled water
Mix these two solutions
Bubble filtered air through solution until color changes (minutes to hours)
Final pH of FeEDTA solution approximately 3.5


Back to Media Top page

Back to Cyanosite Homepage