DCM Medium

TRIS-Base	5.0 g
Sodium Acetate·3H₂O	0.5 g
Monosodium Glutamate	0.5 g
D,L-Glycine	0.5 g
Sucrose	0.5 g
beta-Glycerophosphate	0.4 g
NaNO₃ (75.0 g/L dH₂O)	6.7 ml
Glycerine (100.0 g/L dH₂O)	5.0 ml
NH₄Cl (2.68 g/L dH₂O)	1.0 ml
H₂SeO₃ (1.29 mg/L dH₂O)	1.0 ml
Lactic Acid	1.0 ml
DCM Trace Metal Solution	1.0 ml
Va Vitamin Solution	1.0 ml
Filtered natural seawater to	1.0 L

Solid compounds should be dissolved in dH₂O prior to addition. Adjust pH to 7.6-8.0 (with 3N HCl). Autoclave.

DCM Trace Metal Solution:

Na₂EDTA·2H₂O 29.36 g
FeCl₃·6H₂O 3.15 g
ZnSO₄·7H₂O (22.0 g/L dH₂O) 30.0 ml
MnCl₂·4H₂O (180.0 g/L dH₂O) 20.0 ml
Na₂MoO₄·2H₂O (6.3 g/L dH₂O) 1.0 ml
CoCl₂·6H₂O (10.0 g/L dH₂O) 1.0 ml
CuSO₄·5H₂O (9.8 g/L dH₂O) 0.5 ml
Distilled water to 1.0 L

Va Vitamin Solution:

Inositol 100.0 mg
Thiamine-HCl 20.0 mg
Vitamin B₁₂ (1.0 g/L dH₂O) 0.1 ml
Biotin (0.1 g/L dH₂O) 1.0 ml
Folic Acid (2.0 g/L dH₂O) 0.1 ml
p-Aminobenzoic Acid (2.0 g/L dH₂O) 0.5 ml
Niacin (1.0 g/L dH₂O) 10.0 ml
Ca-Pantothenate (2.0 g/L dH₂O) 10.0 ml
Pyridoxine (1.0 g/L dH₂O) 10.0 ml
Distilled water to 100 ml
Filter sterilize and freeze in plasticware.

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Na₂EDTA·2H₂O	29.36 g
FeCl₃·6H₂O	3.15 g
ZnSO₄·7H₂O (22.0 g/L dH₂O)	30.0 ml
MnCl₂·4H₂O (180.0 g/L dH₂O)	20.0 ml
Na₂MoO₄·2H₂O (6.3 g/L dH₂O)	1.0 ml
CoCl₂·6H₂O (10.0 g/L dH₂O)	1.0 ml
CuSO₄·5H₂O (9.8 g/L dH₂O)	0.5 ml
Distilled water to	1.0 L

Inositol	100.0 mg
Thiamine-HCl	20.0 mg
Vitamin B₁₂ (1.0 g/L dH₂O)	0.1 ml
Biotin (0.1 g/L dH₂O)	1.0 ml
Folic Acid (2.0 g/L dH₂O)	0.1 ml
p-Aminobenzoic Acid (2.0 g/L dH₂O)	0.5 ml
Niacin (1.0 g/L dH₂O)	10.0 ml
Ca-Pantothenate (2.0 g/L dH₂O)	10.0 ml
Pyridoxine (1.0 g/L dH₂O)	10.0 ml
Distilled water to	100 ml