Antia's (ANT) Medium

KNO30.05 g
NaH2PO4·2H2O0.0078 g
Tris1.0 g
Glycine0.3 g
Trace metals stock solution2.5 ml
Thiamine HCl500.0 µg
Cyanocobalamin2.0 µg
Biotin1.0 µg
Filtered natural seawater800.0 ml
Distilled water to1.0 L
Autoclave at 15 psi. Final pH should be 7.6 - 7.8.

Trace metals stock solution:
EDTANa2·2H2O3.24 g
FeCl3·4H2O1.08 g
MnSO4·4H2O0.450 g
ZnSO4·7H2O0.230 g
Na2MoO4·2H2O0.097 g
CuSO4·5H2O0.01 g
CoSO4·7H2O0.0056 g
Distilled water to1.0 L
Adjust pH to 7.6 - 7.8 with dilute HCl or NaOH. Store frozen.


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