Modified SN Medium

Autoclave 1.0 L filtered seawater in teflon lined bottle. Add the following compounds aseptically after autoclaving:

NaNO₃ (300.0 g/L dH₂O) 10.0 ml
K₂HPO₄ (anhydrous) (6.1 g/L dH₂O) 2.5 ml
Na₂EDTA·2H₂O (1.0 g/L dH₂O) 5.0 ml
Na₂CO₃ (4.0 g/L dH₂O) 2.5 ml
H₂SeO₃ (1.29 mg/L dH₂O) 1.0 ml
Va Vitamin Solution 1.0 ml
Cyano Trace Metal Solution 1.0 ml

Cyano Trace Metal Solution:

Citric Acid·H₂O 6.25 g
Ferric Ammonium Citrate 6.0 g
MnCl₂·4H₂O 1.4 g
Na₂MoO₄·2H₂O 0.39 g
Co(NO₃)₂·6H₂O 0.025 g
ZnSO₄·7H₂O 0.222 g
Dissolve each metal compound individually in 100 ml dH₂O. Combine and mix the six solutions, then bring volume up to 1.0 L with dH₂O.

Va Vitamin Solution:

Inositol 100.0 mg
Thiamine-HCl 20.0 mg
Vitamin B₁₂ (1.0 g/L dH₂O) 0.1 ml
Biotin (0.1 g/L dH₂O) 1.0 ml
Folic Acid (2.0 g/L dH₂O) 0.1 ml
p-Aminobenzoic Acid (2.0 g/L dH₂O) 0.5 ml
Niacin (1.0 g/L dH₂O) 10.0 ml
Ca-Pantothenate (2.0 g/L dH₂O) 10.0 ml
Pyridoxine (1.0 g/L dH₂O) 10.0 ml
Distilled water to 100 ml
Filter sterilize and freeze in plasticware.

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NaNO₃ (300.0 g/L dH₂O)	10.0 ml
K₂HPO₄ (anhydrous) (6.1 g/L dH₂O)	2.5 ml
Na₂EDTA·2H₂O (1.0 g/L dH₂O)	5.0 ml
Na₂CO₃ (4.0 g/L dH₂O)	2.5 ml
H₂SeO₃ (1.29 mg/L dH₂O)	1.0 ml
Va Vitamin Solution	1.0 ml
Cyano Trace Metal Solution	1.0 ml

Citric Acid·H₂O	6.25 g
Ferric Ammonium Citrate	6.0 g
MnCl₂·4H₂O	1.4 g
Na₂MoO₄·2H₂O	0.39 g
Co(NO₃)₂·6H₂O	0.025 g
ZnSO₄·7H₂O	0.222 g

Inositol	100.0 mg
Thiamine-HCl	20.0 mg
Vitamin B₁₂ (1.0 g/L dH₂O)	0.1 ml
Biotin (0.1 g/L dH₂O)	1.0 ml
Folic Acid (2.0 g/L dH₂O)	0.1 ml
p-Aminobenzoic Acid (2.0 g/L dH₂O)	0.5 ml
Niacin (1.0 g/L dH₂O)	10.0 ml
Ca-Pantothenate (2.0 g/L dH₂O)	10.0 ml
Pyridoxine (1.0 g/L dH₂O)	10.0 ml
Distilled water to	100 ml